You cannot have RAID 0 on a single disk, so the OS disk will be a simple disk volume on a single disk.
You do not mention what operating system you are using or whether the computer has a RAID controller. I am going to assume you want to set this up as software raid under XP.
1. Convert the two disks for RAID to Dynamic Disks.
2. Set up a RAID set on the first disk.
3. Add the second disk.
I am including a link for a tutorial. There are a ton of other tutorials online to describe the process.
Good luck!